General description

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. Optimal pH: 9.0-9.5.

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

Packaging

2 u in Glass bottle

Reconstitution

Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Unit Definition

One AU (amidase unit) is defined as the amount of enzyme that catalyzes the release of 1 µM pNA from N-benzoyl-DL-lysine-pNA per min at 30°C, pH 9.5.

Warning

Toxicity: Standard Handling (A)